UJI TOKSISITAS DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) DAN IDENTIFIKASI KOMPONEN FITOKIMIA EKSTRAK ASETON KULIT BUAH KAKAO (Theobroma cacao L.)

Authors

  • Dewi Chusniasih
  • Tutik Tutik

DOI:

https://doi.org/10.23960/aec.v5i2.2020.p192-201

Abstract

Uji toksisitas dan identifikasi komponen fitokimia ekstrak aseton kulit buah kakao (Theobroma cacao L) telah dilakukan. Sampel kulit buah kakao diekstraksi dengan metode maserasi dengan pelarut aseton 80%, pengujian toksisitas dilakukan dengan metode Brine Shrimp Lethality Test (BSLT), dan ekstrak dianalisis dengan metode Gas Chromatography – Mass Spectrofotometry (GC-MS). Ekstrak aseton kulit buah kakao bersifat toksik berdasarkan uji toksisitas dengan metode BSLT dengan nilai LC50 yaitu 24,69 ppm. Hasil analisis GC-MS diperoleh bahwa kandungan fitokimia ekstrak aseton kulit buah kakao didominasi oleh golongan asam lemak metil ester, yaitu sebanyak 15 senyawa dengan total persentase kelimpahan 93,94%. Senyawa dengan kelimpahan tertinggi yaitu n-asam heksadekanoat dengan luas area 31,02%, asam oleat dengan luas area 20,27%, dan asam oktadekanoat dengan luas area 12,24%.

http://dx.doi.org/10.23960/aec.v5.i2.2020.p192-201 

Downloads

Published

2020-10-30

How to Cite

Chusniasih, D., & Tutik, T. (2020). UJI TOKSISITAS DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) DAN IDENTIFIKASI KOMPONEN FITOKIMIA EKSTRAK ASETON KULIT BUAH KAKAO (Theobroma cacao L.). Analit : Analytical and Environmental Chemistry, 5(02), 192–201. https://doi.org/10.23960/aec.v5i2.2020.p192-201

Issue

Vol. 5, No. 02 October (2020) Analit : Analytical and Environmental Chemistry

Section

Articles

License

 

Authors who publish with this journal agree to the following terms:
  1. Authors retain copyright and acknowledge that the Analit : Analytical and Environmental Chemistry  is the first publisher, licensed under a Creative Commons Attribution 4.0 International License.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges and earlier and greater citation of published work.